Sample Set Annotation Tool: The human placental sexome differs between trophoblast epithelium and villous vessel endothelium

Study Design
Assay
Sample Info
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Analysis

Purpose

As susceptibility to many adult disorders originates in utero, we here hypothesized that fetal sex influences gene expression in placental cells and produces functional differences in human placentas. We found that fetal sex differentially affects gene expression in a cell-phenotype dependent manner among all four placental cell-phenotypes studied: cytotrophoblasts, syncytiotrophoblasts, arterial endothelial cells and venous endothelial cells. The markedly enriched pathways in males were identified to be signaling pathways for graft-versus-host disease as well as the immune and inflammatory systems, both supporting the hypothesis that there is reduced maternal-fetal compatibility for male fetuses.Our study is the first microarray study investigating sexual dimorphism in purified and characterized somatic cells from a single human tissue, the placenta, that underlines the importance of considering fetal sex as an independent variable in any work using human placenta.

Hypothesis

Experimental Design

Arterial and venous endothelial cells were isolated from eight different placentas, four of each sex. A total of ten placentas were used for isolation of cytotrophoblasts and six for syncytiotrophoblasts, with equal numbers from each sex.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

32 Samples Loaded: 32

Species

Human (Homo sapiens)

Sample Source

placenta

Disease

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Sample ID	!Sample_title	tissue	cell type	gender	!Sample Title
GSM1083974	CT_58_female_20O2_VitDctrl	placenta	cytotrophoblasts	female	CT 58 female 20O2 VitDctrl
GSM1083975	CT_60_female_20O2_VitDctrl	placenta	cytotrophoblasts	female	CT_60_female_20O2_VitDctrl
GSM1083976	CT_66_male_20O2_VitDctrl	placenta	cytotrophoblasts	male	CT_66_male_20O2_VitDctrl
GSM1083977	CT_81_male_20O2_VitDctrl	placenta	cytotrophoblasts	male	CT_81_male_20O2_VitDctrl
GSM1083978	CT_82_male_20O2_VitDctrl	placenta	cytotrophoblasts	male	CT_82_male_20O2_VitDctrl

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File
sampleset4000015_sampleannotations_group.csv Sample Set Spreadsheet

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Sample ID	!Sample Title	Tissue	Cell type	Gender	!Sample Title	Group	Cell type
GSM1083974	CT_58_female_20O2_VitDctrl	placenta	cytotrophoblasts	female	CT 58 female 20O2 VitDctrl	cytotrophoblasts female	cytotrophoblasts
GSM1083975	CT_60_female_20O2_VitDctrl	placenta	cytotrophoblasts	female	CT_60_female_20O2_VitDctrl	cytotrophoblasts female	cytotrophoblasts
GSM1083976	CT_66_male_20O2_VitDctrl	placenta	cytotrophoblasts	male	CT_66_male_20O2_VitDctrl	cytotrophoblasts male	cytotrophoblasts
GSM1083977	CT_81_male_20O2_VitDctrl	placenta	cytotrophoblasts	male	CT_81_male_20O2_VitDctrl	cytotrophoblasts male	cytotrophoblasts
GSM1083978	CT_82_male_20O2_VitDctrl	placenta	cytotrophoblasts	male	CT_82_male_20O2_VitDctrl	cytotrophoblasts male	cytotrophoblasts
GSM1083979	CT_84m_male_20O2_VitDctrl	placenta	cytotrophoblasts	male	CT_84m_male_20O2_VitDctrl	cytotrophoblasts male	cytotrophoblasts
GSM1083980	CT_87_female_20O2_VitDctrl	placenta	cytotrophoblasts	female	CT_87_female_20O2_VitDctrl	cytotrophoblasts female	cytotrophoblasts
GSM1083981	CT_90_male_20O2_VitDctrl	placenta	cytotrophoblasts	male	CT_90_male_20O2_VitDctrl	cytotrophoblasts male	cytotrophoblasts
GSM1083982	CT_91_female_20O2_VitDctrl	placenta	cytotrophoblasts	female	CT_91_female_20O2_VitDctrl	cytotrophoblasts female	cytotrophoblasts
GSM1083983	CT_93_female_20O2_VitDctrl	placenta	cytotrophoblasts	female	CT_93_female_20O2_VitDctrl	cytotrophoblasts female	cytotrophoblasts
GSM1083984	SCT_81_male_20O2_VitDctrl	placenta	syncytiotrophoblasts	male	SCT_81_male_20O2_VitDctrl	syncytiotrophoblasts male	syncytiotrophoblasts
GSM1083985	SCT_82_male_20O2_VitDctrl	placenta	syncytiotrophoblasts	male	SCT_82_male_20O2_VitDctrl	syncytiotrophoblasts male	syncytiotrophoblasts
GSM1083986	SCT_84m_male_20O2_VitDctrl	placenta	syncytiotrophoblasts	male	SCT_84m_male_20O2_VitDctrl	syncytiotrophoblasts male	syncytiotrophoblasts
GSM1083987	SCT_87_female_20O2_VitDctrl	placenta	syncytiotrophoblasts	female	SCT_87_female_20O2_VitDctrl	syncytiotrophoblasts female	syncytiotrophoblasts
GSM1083988	SCT_91_female_20O2_VitDctrl	placenta	syncytiotrophoblasts	female	SCT_91_female_20O2_VitDctrl	syncytiotrophoblasts female	syncytiotrophoblasts
GSM1083989	SCT_93_female_20O2_VitDctrl	placenta	syncytiotrophoblasts	female	SCT_93_female_20O2_VitDctrl	syncytiotrophoblasts female	syncytiotrophoblasts
GSM1083990	AEC_105_female_21O2_VitDctrl	placenta	arterial endothelial cells	female	AEC_105_female_21O2_VitDctrl	arterial endothelial cells female	arterial endothelial cells
GSM1083991	AEC_127_female_21O2_VitDctrl	placenta	arterial endothelial cells	female	AEC_127_female_21O2_VitDctrl	arterial endothelial cells female	arterial endothelial cells
GSM1083992	AEC_135_female_21O2_VitDctrl	placenta	arterial endothelial cells	female	AEC_135_female_21O2_VitDctrl	arterial endothelial cells female	arterial endothelial cells
GSM1083993	AEC_84f_female_21O2_VitDctrl	placenta	arterial endothelial cells	female	AEC_84f_female_21O2_VitDctrl	arterial endothelial cells female	arterial endothelial cells
GSM1083994	VEC_105_female_21O2_VitDctrl	placenta	venous endothelial cells	female	VEC_105_female_21O2_VitDctrl	venous endothelial cells female	venous endothelial cells
GSM1083995	VEC_127_female_21O2_VitDctrl	placenta	venous endothelial cells	female	VEC_127_female_21O2_VitDctrl	venous endothelial cells female	venous endothelial cells
GSM1083996	VEC_135_female_21O2_VitDctrl	placenta	venous endothelial cells	female	VEC_135_female_21O2_VitDctrl	venous endothelial cells female	venous endothelial cells
GSM1083997	VEC_84f_female_21O2_VitDctrl	placenta	venous endothelial cells	female	VEC_84f_female_21O2_VitDctrl	venous endothelial cells female	venous endothelial cells
GSM1083998	VEC_100_male_21O2_VitDctrl	placenta	venous endothelial cells	male	VEC_100_male_21O2_VitDctrl	venous endothelial cells male	venous endothelial cells
GSM1083999	AEC_117_male_21O2_VitDctrl	placenta	arterial endothelial cells	male	AEC_117_male_21O2_VitDctrl	arterial endothelial cells male	arterial endothelial cells
GSM1084000	AEC_92_male_21O2_VitDctrl	placenta	arterial endothelial cells	male	AEC_92_male_21O2_VitDctrl	arterial endothelial cells male	arterial endothelial cells
GSM1084001	AEC_98_male_21O2_VitDctrl	placenta	arterial endothelial cells	male	AEC_98_male_21O2_VitDctrl	arterial endothelial cells male	arterial endothelial cells
GSM1084002	AEC_100_male_21O2_VitDctrl	placenta	arterial endothelial cells	male	AEC_100_male_21O2_VitDctrl	arterial endothelial cells male	arterial endothelial cells
GSM1084003	VEC_117_male_21O2_VitDctrl	placenta	venous endothelial cells	male	VEC_117_male_21O2_VitDctrl	venous endothelial cells male	venous endothelial cells
GSM1084004	VEC_92_male_21O2_VitDctrl	placenta	venous endothelial cells	male	VEC_92_male_21O2_VitDctrl	venous endothelial cells male	venous endothelial cells
GSM1084005	VEC_98_male_21O2_VitDctrl	placenta	venous endothelial cells	male	VEC_98_male_21O2_VitDctrl	venous endothelial cells male	venous endothelial cells

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