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Clinical
Placentas delivered by term spontaneous labour compared to those delivered by elective term Cesarean section - GSE18850
Purpose

Differentially expressed mRNA transcripts in the placenta delivered by term spontaneous labour compared to those delivered by elective term cesarean section.We hypothesized that the labour process involves changes in mRNA expression in the placenta. To test this hypothesis, we interrogated the mRNA levels of >50,000 genes and transcript variants using gene expression microarray (Human Genome U133 Plus 2.0 Array, Affymetrix) on 5 placentas collected from term spontaneous delivery and another 5 placentas collected from elective term cesarean delivery. To minimize the effect of gestational age on gene expression, these two groups of placentas were matched for their gestational ages at delivery.We have identified 134 and 128 genes that were up- or down-regulated, respectively, for more than 3-fold in the term (spontaneous) labour placentas compared to the term (elective) cesarean placentas (Mann-Whitney Rank Sum Test, p-value <= 0.05 after Benjamini and Hochberg adjustment for multiple testing).

Hypothesis

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Experimental Design

Placentas collected from (i) term spontaeous labour and (ii) elective term cesarean section were subjected to RNA extraction and hybridization on Affymetrix microarrays. To identify gene expression patterns that are commonly involved in term spontaneous labour, we analyzed 5 placentas from each of these 2 groups and tested for any differentially expressed genes by non-parametric statistical methods.

Experimental Variables

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Controls

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Methods

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Additional Information

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Microarray
Affymetrix HG-U133_Plus_2
10 Samples Loaded: 10
Human (Homo sapiens)
placenta
Sample Set Spreadsheet
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Samples Preview
Sample ID tissue !Sample Title Group Delivery location Ethinicity of pregnant mother
GSM467138 placenta Placenta, Term labour spontaneous delivery, Biological Replicate #1 labour Prince of Wales Hospital, Hong Kong Chinese
GSM467139 placenta Placenta, Term labour spontaneous delivery, Biological Replicate #2 labour Prince of Wales Hospital, Hong Kong Chinese
GSM467140 placenta Placenta, Term labour spontaneous delivery, Biological Replicate #3 labour Prince of Wales Hospital, Hong Kong Chinese
GSM467141 placenta Placenta, Term labour spontaneous delivery, Biological Replicate #4 labour Prince of Wales Hospital, Hong Kong Chinese
GSM467142 placenta Placenta, Term labour spontaneous delivery, Biological Replicate #5 labour Prince of Wales Hospital, Hong Kong Chinese
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sampleset4000029_sampleannotations_group.csv
Sample Set Spreadsheet
sampleset4000029_sampleannotations_group.csv
Sample Set Spreadsheet
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Sample ID Tissue !Sample Title Group Delivery location Ethinicity of pregnant mother
GSM467138
placenta
Placenta, Term labour spontaneous delivery, Biological Replicate #1
labour
Prince of Wales Hospital, Hong Kong
Chinese
GSM467139
placenta
Placenta, Term labour spontaneous delivery, Biological Replicate #2
labour
Prince of Wales Hospital, Hong Kong
Chinese
GSM467140
placenta
Placenta, Term labour spontaneous delivery, Biological Replicate #3
labour
Prince of Wales Hospital, Hong Kong
Chinese
GSM467141
placenta
Placenta, Term labour spontaneous delivery, Biological Replicate #4
labour
Prince of Wales Hospital, Hong Kong
Chinese
GSM467142
placenta
Placenta, Term labour spontaneous delivery, Biological Replicate #5
labour
Prince of Wales Hospital, Hong Kong
Chinese
GSM467143
placenta
Placenta, Term cesarean delivery, Biological Replicate #1
cesarean
Prince of Wales Hospital, Hong Kong
Chinese
GSM467144
placenta
Placenta, Term cesarean delivery, Biological Replicate #2
cesarean
Prince of Wales Hospital, Hong Kong
Chinese
GSM467145
placenta
Placenta, Term cesarean delivery, Biological Replicate #3
cesarean
Prince of Wales Hospital, Hong Kong
Chinese
GSM467146
placenta
Placenta, Term cesarean delivery, Biological Replicate #4
cesarean
Prince of Wales Hospital, Hong Kong
Chinese
GSM467147
placenta
Placenta, Term cesarean delivery, Biological Replicate #5
cesarean
Prince of Wales Hospital, Hong Kong
Chinese

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