Sample Set Annotation Tool: Transcriptomic Analysis of Placenta Affected by Antiphospholipid Antibodies: Following the TRAIL of trophoblast death

Study Design
Assay
Sample Info
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Info
Samples
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Analysis

Purpose

Antiphospholipid antibodies, a maternal risk factor for preeclampsia, increase shedding of necrotic trophoblast debris from the placenta, leading to endothelial dysfunction. Using Affymetrix HGU133 Plus 2 microarrays, we found changes in the transcriptome of placental explants treated with antiphospholipid antibodies including seven mRNAs encoding for genes BCL2L1, MCL1, PDCD2L, FASLG, SEMA6A, PRKCE and TRAIL that are involved in the regulation of apoptosis. Quantitative real-time RT-PCR and immunohistochemistry confirmed a reduction in TRAIL expression. These results may help to understand how antiphospholipid antibodies affect trophoblast cell death and how the antibodies could contribute to the pathogenesis of preeclampsia.

Hypothesis

Experimental Design

Three different first-trimester placentas (8-8.5 weeks gestation) were dissected into 6 explants each and cultured either with antiphospholipid antibodies (25ug/mL) or untreated media for 16h. RNA was extracted from treated placental explants and Affymetrix HGU133 Plus 2 arrays were conducted to investigate changes in mRNA expression.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

6 Samples Loaded: 6

Species

Human (Homo sapiens)

Sample Source

placenta

Disease

Treatment Protocol

Antiphospholipid antibody

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	tissue	gestation	treatment
GSM880866	plac_unt_1	placenta	8 weeks	none
GSM880867	plac_apl_1	placenta	8 weeks	antiphospholipid antibody
GSM880868	plac_unt_2	placenta	8.1 weeks	none
GSM880869	plac_apl_2	placenta	8.1 weeks	antiphospholipid antibody
GSM880870	plac_unt_3	placenta	8.5 weeks	none

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Sample ID	!Sample Title	Tissue	Gestation	Treatment
GSM880866	plac_unt_1	placenta	8 weeks	none
GSM880867	plac_apl_1	placenta	8 weeks	antiphospholipid antibody
GSM880868	plac_unt_2	placenta	8.1 weeks	none
GSM880869	plac_apl_2	placenta	8.1 weeks	antiphospholipid antibody
GSM880870	plac_unt_3	placenta	8.5 weeks	none
GSM880871	plac_apl_3	placenta	8.5 weeks	antiphospholipid antibody

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