Gene expression profiling of trophoblast cells - GSE9773
Invasion of cytotrophoblasts into uterine tissues is essential for placental development. To identify molecules regulating trophoblast invasion, mRNA signatures of purified ...
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To identify genes potentially regulating cell invasion trophoblast cells of early human gestation with distinct invasive properties were profiled.Distinct gene expression ...
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| Sample ID | !Sample Title | Group | Dissected mesenchymal villous tissue of early pregnancy was arranged radially on 80 μl drops of growth-factor-reduced Matrigel™ (BD, Bedford, MA) in 24 well plates (SFM; DMEM | Trophoblasts were cultured for 24 h in DMEM (GIBCO) | With 10% FCS, 100 mg/ml streptomycin and 100 IU/ml penicillin |
|---|---|---|---|---|---|
| GSM246492 | EVT-1 | extravillous trophoblasts | Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. | ||
| GSM246493 | EVT-2 | extravillous trophoblasts | Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. | ||
| GSM246494 | EVT-3 | extravillous trophoblasts | Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. | ||
| GSM246495 | CTB-1 | cytotrophoblasts | TRUE | TRUE | |
| GSM246497 | CTB-2 | cytotrophoblasts | TRUE | TRUE |
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| Sample ID | !Sample Title | Group | Dissected mesenchymal villous tissue of early pregnancy was arranged radially on 80 μl drops of growth-factor-reduced Matrigel™ (BD, Bedford, MA) in 24 well plates (SFM; DMEM | Trophoblasts were cultured for 24 h in DMEM (GIBCO) | With 10% FCS, 100 mg/ml streptomycin and 100 IU/ml penicillin |
|---|---|---|---|---|---|
| GSM246492 | EVT-1 |
extravillous trophoblasts |
Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. |
||
| GSM246493 | EVT-2 |
extravillous trophoblasts |
Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. |
||
| GSM246494 | EVT-3 |
extravillous trophoblasts |
Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. |
||
| GSM246495 | CTB-1 |
cytotrophoblasts |
TRUE |
TRUE |
|
| GSM246497 | CTB-2 |
cytotrophoblasts |
TRUE |
TRUE |
|
| GSM246499 | CTB-3 |
cytotrophoblasts |
TRUE |
TRUE |
|
| GSM307639 | EVT-4 |
extravillous trophoblasts |
Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. |
||
| GSM307640 | EVT-5 |
extravillous trophoblasts |
Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation. |
||
| GSM307641 | EVT-6 |
extravillous trophoblasts |
Ham's F12 (Gibco) 1:1, supplemented with 100 µg/ml streptomycin/penicillin (Gibco) and incubated for 4 hours at 37°C, 5% CO2 to allow attachment. Wells were then carefully flooded with 500 µl SFM, incubated at 37°C, 5% CO2 and subsequent growth characteristics were monitored by light microscopy. Total RNA or tissue sections were prepared after 72 hours of incubation |
||
| GSM307642 | CTB-4 |
cytotrophoblasts |
TRUE |
TRUE |
|
| GSM307643 | CTB-5 |
cytotrophoblasts |
TRUE |
TRUE |
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